Simplified basic media for human pluripotent cell culture

摘要

Fully defined media that support pluripotent cell viability, proliferation, cloning, and derivation, as well as methods and compositions including these media are described. Methods for deriving iPS cells from adult individuals under defined, xeno-free conditions are also described.

主权项

1. A defined, albumin-free medium free of any component obtained from a non-human animal, the medium comprising water, salts, amino acids, vitamins, glucose, insulin, an FGF, selenium, transferrin, and one of TGF-β and NODAL, each in an amount sufficient to support human pluripotent stem cell proliferation.