High-cell density fed-batch fermentation process for producing recombinant protein

摘要

Methods for producing proteins, for example, recombinant meningococcal 2086 proteins, using fed-batch fermentation with continuous input of an inducer after achieving a threshold parameter, and optionally continuous input of a carbon source, for example, a constant rate input, to improve protein yields, as well as high density protein compositions and compositions for use in the methods of the present invention, are provided.

主权项

1. A method for producing a recombinant protein comprising culturing a recombinant bacterial cell to express a recombinant protein in a culture; continuously adding a carbon source to the culture; continuously adding arabinose to the culture after the culture achieves a threshold parameter, said arabinose being added to the culture simultaneously to the continuous addition of the carbon source; and isolating the recombinant protein from the culture, wherein the carbon source feed rate during the arabinose induction is between 2.25 and 7.5 g/L/h; and wherein the arabinose feed rate is between 1.7 and 6.7 g/L/h.