Processes for detecting or quantifying analytes of interest

摘要

This invention provides novel compositions and processes for analyte detection, quantification and amplification. Nucleic acid arrays and libraries of analytes are usefully incorporated into such compositions and processes. Universal detection elements, signaling entities and the like are employed to detect and if necessary or desirable, to quantify analytes. Amplification of target analytes are also provided by the compositions and processes of this invention.

主权项

1. A process for detecting or quantifying non-nucleic acid analytes of interest, said process comprising the steps of:
1) providing
a) an array on a solid surface comprising a plurality of discrete areas; wherein
at least two of said discrete areas each comprise a nucleotide sequence fixed or immobilized to said discrete areas,said at least two discrete areas comprise a first discrete area and a second discrete area, andsaid nucleotide sequence on said first discrete area is different from said nucleotide sequence on said second discrete area;b) chimeric compositions consisting of:
i) a nucleic acid portion; andii) a non-nucleic acid portion;wherein
said nucleic acid portion of each chimeric composition is complementary to a nucleotide sequence of said array,said nucleic acid portion of the chimeric composition that is complementary to the nucleotide sequence located at said first discrete area has a different sequence from that of said nucleic acid portion of the chimeric composition complementary to the nucleotide sequence located at said second discrete area,said non-nucleic acid portion of the chimeric composition that has a nucleic acid portion that is complementary to the nucleotide sequence of said first discrete area has a binding affinity for a first non-nucleic acid analyte of interest and said non-nucleic acid portion of the chimeric composition that has a nucleic acid portion that is complementary to the nucleotide sequence of said second discrete area has a binding affinity for a second non-nucleic acid analyte of interest,said first non-nucleic acid analyte is different from said second non nucleic acid analyte,when said non-nucleic acid portion of a chimeric composition is a peptide or protein, said nucleic acid portion of said chimeric composition does not comprise a sequence which is either identical or complementary to a sequence that codes for said peptide or protein, andsaid nucleic acid portion i) and said non-nucleic acid portion ii) are covalently attached to each other;c) a sample containing or suspected of containing said non-nucleic acid analytes of interest, wherein
said non-nucleic acid analytes of interest comprise oligopeptides, polypeptides, oligosaccharides, polysaccharides, lipids, ligands, or combinations thereof; andd) signal generating means; 2) contacting said array with said chimeric compositions to hybridize the nucleic acid portions of said chimeric compositions to complementary nucleotide sequences fixed or immobilized to said array; 3) contacting said array with the sample under conditions permissive of binding said non-nucleic acid analytes to said non-nucleic acid portion; 4) contacting said bound non-nucleic acid analytes with said signal generating means; and 5) detecting or quantifying the presence of said non-nucleic acid analytes by measuring said signal generating means bound to said array.