Water-soluble AIE luminogens for monitoring and retardation of fibrillation of amyloid proteins

摘要

Compounds that exhibit aggregation induced emission (AIE), and more particularly to water-soluble conjugated polyene compounds that exhibit aggregation induced emission. The conjugated polyene compounds can be used as bioprobes for DNA detection, G-quadruplex identification, and potassium-ion sensing. The polyenes also can be utilized as an external fluorescent marker to study conformational structures, to monitor folding processes of label-free oligonucleotides with G-rich strand sequences, and to visualize DNA bands in PAGE assay. The polyenes have applications in high-throughput anticancer drug screening and are useful for the development of efficient anti-cancer drugs. Furthermore, the present subject matter can also be used to monitor fibrillation of amyloid proteins and to facilitate the storage and delivery thereof.

主权项

1. A method of monitoring amyloid protein fibrillation in a biological sample comprising contacting a water-soluble conjugated polyene compound that exhibits aggregation induced emission with the biological sample containing an amyloid protein; and detecting fluorescence emitted by said water-soluble conjugated polyene compound and the fluorescence emitted is induced by aggregation of said water-soluble conjugated polyene compound when contacting with said biological sample, wherein said water-soluble conjugated polyene compound is selected from the group consisting of:
1,2-Bis[4-(3-sulfonatopropoxy)phenyl]-1,2-diphenylethene sodium; 1,2-Bis[4-(3-triphenylphosphonium)phenyl]-1,2-diphenylethene bromide; 1-[4-(1-methyl-4-pyridine)ethene]-1,2,3-triphenylethene hexafluorophosphate; 1-[4-(1,2-dimethyl-5-benzothiazole)]-1,2,3-triphenylethene iodide; N,N′,N″,N′″-[1,2-tetrakis(1,4-phenoxyethyl)vinyl]tetrakis(trimethylammoniumbromide); 1-[4-(1-ethyl-2-benzothiazole)ethene]-1,2,3-triphenylethene hexafluorophosphate; and 1,1′-methyl-2,5-bis[4-(1-sulfonatopropyl-2-isoindole)ethenephenyl]-3,4-bisphenyl silole.