SYSTEMS AND METHODS FOR GENOTYPING SEED COMPONENTS

摘要

The invention provides methods for obtaining genetic material from plant embryos while preserving their viability as well as methods for performing a molecular analysis of plant embryos, particularly with small quantities of genetic material. The methods may include the steps of collecting shed cellular material from one or more plant embryos; obtaining DNA from the shed cellular material; performing a molecular analysis of the DNA; and germinating at least one of said one or more plant embryos. A further extension of this method includes determining whether to germinate and grow the embryo or to discard the embryo based on its genotype as part of a breeding process. Also provided are methods of genotyping embryos using embryo shed cellular material contained in or on agar and methods of analyzing plant embryonic tissue derived from microspores.

主权项

1. A method of analyzing a plant embryo comprising:
a. obtaining a portion of agar in contact with a plant embryo, wherein said portion comprises shed cellular material of said plant embryo; b. obtaining genetic material from the shed cellular material of the plant embryo; and c. performing a molecular analysis of the genetic material obtained from said shed cellular material of the plant embryo.