RAPID SALMONELLA SEROTYPING ASSAY

摘要

Processes for the serotype specific detection and identification of one or more Salmonella serotypes are provided. A family of specific primers and probes are provided that allow screening of biological or environmental samples for robust, rapid, and reproducible detection and identification of one or more Salmonella serotypes in the sample.

主权项

1. A process of specifically identifying Salmonella ser. Enteritidis in a sample comprising:
performing a first stage assay by adding to a sample a plurality of SE primer sets operable to produce amplification products of SE target sequences, said primer sets each comprising a forward primer that hybridizes to a region flanking a target sequence under conditions suitable for a polymerase chain reaction, and a reverse primer that hybridizes to a different region of the corresponding target sequence under conditions suitable for a polymerase chain reaction, wherein each target sequence is a portion of a Salmonella gene; detecting the presence of or absence of each of said amplification products by hybridization of a probe specific to said detected amplification product.