DENDRITIC CELL TUMOR VACCINE AND METHOD FOR PREPARING THE SAME

摘要

A method of preparing the dendritic cell tumor vaccine includes steps as follows. A tumor specimen is primarily isolated and cultured to obtain a plurality of tumor cells. Cancer stem cells having a specific cell surface marker are sorted from the tumor cells. The cancer stem cells are irradiated with a radiation. A plurality of dendritic cells are provided. The dendritic cells and the cancer stem cells irradiated with the radiation are co-cultured for activating the dendritic cells into cancer-stem-cell-antigen-presenting dendritic cells to obtain the dendritic cell tumor vaccine. The dendritic cell tumor vaccine is a mixture of the cancer-stem-cell-antigen-presenting dendritic cells and the cancer stem cells.

主权项

1. A method of preparing a dendritic cell tumor vaccine, comprising:
(a) primarily isolating and culturing a tumor specimen to obtain a plurality of tumor cells; (b) sorting cancer stem cells having a specific cell surface marker from the tumor cells; (c) irradiating the cancer stem cells with a radiation; (d) providing a plurality of dendritic cells; and (e) co-culturing the dendritic cells and the cancer stem cells irradiated with the radiation for activating the dendritic cells into cancer-stem-cell-antigen-presenting dendritic cells to obtain the dendritic cell tumor vaccine, wherein the dendritic cell tumor vaccine is a mixture of the cancer-stem-cell-antigen-presenting dendritic cells and the cancer stem cells.