发明名称 |
Genome modification using guide polynucleotide/Cas endonuclease systems and methods of use |
摘要 |
Compositions and methods are provided for genome modification of a target sequence in the genome of a cell. The methods and compositions employ a guide polynucleotide/Cas endonuclease system to provide an effective system for modifying or altering target sites within the genome of a cell or organism. Once a genomic target site is identified, a variety of methods can be employed to further modify the target sites such that they contain a variety of polynucleotides of interest. Compositions and methods are also provided for editing a nucleotide sequence in the genome of a cell. Breeding methods and methods for selecting plants utilizing a two component RNA polynucleotide and Cas endonuclease system are also disclosed. |
申请公布号 |
AU2014308898(A1) |
申请公布日期 |
2016.03.03 |
申请号 |
AU20140308898 |
申请日期 |
2014.08.20 |
申请人 |
PIONEER HI-BRED INTERNATIONAL, INC. |
发明人 |
CIGAN, ANDREW;PATTEN, PHILLIP A.;YOUNG, JOSHUA K. |
分类号 |
C12N15/113;C07K14/315;C12N15/115;C12N15/82 |
主分类号 |
C12N15/113 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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