METHOD FOR MEASUREMENT OF THROMBOCYTE FUNCTION

摘要

With a method for measurement of thrombocyte function, a solution is created, by which the sensitivity of individual thrombocytes can be measured with the least possible apparatus effort, with high throughput, by passing a liquid thrombocyte solution, in which the thrombocytes are present in isolated form, into a microfluidic chamber and brought into contact with at least one stimulant, wherein an electrical field directed transverse to the entry direction of the thrombocyte solution is applied to the chamber, and the movement path of the thrombocytes in the electrical field is observed and evaluated, in such a manner that thrombocytes having a movement path directed in the direction toward the minus pole of the electrical field are classified as non-activated thrombocytes, and thrombocytes having a movement path directed in the direction toward the plus pole of the electrical field are classified as activated thrombocytes.

主权项

1. A method for measurement of thrombocyte function comprising
(a) passing a liquid thrombocyte solution comprising thrombocytes in isolated form into a microfluidic chamber; (b) applying to the microfluidic chamber an electrical field directed transverse to an entry direction of the thrombocyte solution; (c) bringing the thrombocyte solution into contact with at least one stimulant directly before or during action of the electrical field; and (d) observing and evaluating a respective movement path of each individual thrombocyte in the electrical field so that a thrombocyte having a movement path directed toward a minus pole of the electrical field is classified as an activated thrombocyte and a thrombocyte having a movement path directed toward a plus pole of the electrical field is classified as a non-activated thrombocyte.