LIPOPEPTIDES FOR DELIVERY OF NUCLEIC ACIDS

摘要

Lipopeptide compounds having a central peptide and a lipophilic group attached to at least one terminus of the peptide, or to at least one amino acid residue of the peptide, and salts and uses thereof. The lipophilic group may be attached to the N-terminus, C-terminus or both termini of the peptide. The lipophilic group may be attached to at least one interal amino acid residue. The lipophilic group may be attached to either termini or both and at least one internal amino acid residue.

主权项

1. A compound having the structure shown in Formula I:
R3-J—{NRN—CR1R2—(C═O)}n—Z—R4  Formula I wherein
R′ is independently, for each occurrence, a non-hydrogen side chain of an amino acid;{NRN—CR1R2—(C═O)}n is a central peptide; R2 and RN are independently of one another hydrogen, or an organic group consisting of carbon, oxygen, nitrogen, sulfur, and hydrogen atoms, and having from 1 to 20 carbon atoms, or C(1-22)alkyl, (6-12)cycloalkyl, (6-12)cycloalkylalkyl, C(3-18)alkenyl, C(3-18)alkynyl, C(1-5)alkanoyl, C(1-5)alkanoyloxy, C(1-5)alkoxy, C(1-5)alkoxy-C(1-5)alkyl, C(1-5) alkoxy-C(1-5)alkoxy, C(1-5)alkyl-amino-C(1-5)alkyl-, C(1-5)dialkyl-amino-C(1-5)alkyl-, nitro-C(1-5)alkyl, cyano-C(1-5)alkyl, aryl-C(1-5)alkyl, 4-biphenyl-C(1-5)alkyl, carboxyl, or hydroxyl; R3 and R4 are independently of one another, a lipophilic tail derived from a naturally-occurring or synthetic lipid, phospholipid, glycolipid, triacylglycerol, glycerophospholipid, sphingolipid, ceramide, sphingomyelin, cerebroside, or ganglioside, wherein the tail may contain a steroid, or an organic group consisting of carbon, oxygen, nitrogen, sulfur, and hydrogen atoms, and having from 1 to 20 carbon atoms, or a substituted or unsubstituted C(1-22)alkyl, C(6-12)cycloalkyl, C(6-12)cycloalkyl-alkyl, C(3-18)alkenyl, C(3-18)alkynyl, C(1-5)alkoxy-C(1-5)alkyl, or a sphinganine, (2R,3R)-2-amino-1,3-octadecanediol, icosasphinganine, sphingosine, phytosphingosine, cis-4-sphingenine, or a ceramide; Z is NH, O, or a linker comprising a maleimido, thioether, amide, cysteamide, cysteine, thiol, or a disulfide group, or a polyethyleneoxide or polypropyleneoxide group comprising 1-400 atoms, or a linker comprising 1-200 atoms selected from the group of C, H, F, Cl, Br, N, O, S, Si, and P;
J is (C═O), O, or a linker comprising a maleimido, thioether, amide, cysteamide, cysteine, thiol, or disulfide group, or a polyethyleneoxide or polypropyleneoxide group comprising 1-400 atoms, or a linker comprising 1-200 atoms selected from the group of C, H, F, Cl, Br, N, O, S, Si, and P; and wherein
n is 2 to 100.