SYSTEMS AND METHODS FOR PERICARP GENOTYPING

摘要

The invention includes methods for obtaining samples of maternal tissue from seeds, obtaining genetic material from the maternal seed tissue, and performing a molecular analysis on the genetic material from the maternal seed tissue to determine maternal lineage of a single seed. The invention also includes methods for establishing a consensus maternal genotype from maternal seed tissues obtained from multiple seeds as well as methods for determining paternal lineage.

主权项

1. A method for determining maternal lineage of one or more seeds, said method comprising:
(a) collecting maternal seed tissue from the one or more seeds; (b) washing the maternal seed tissue; (c) dissociating and homogenizing the maternal seed tissue to obtain a homogenized solution; (d) centrifuging the homogenized solution obtained in step (c) to obtain supernatant; and (e) performing a molecular analysis on the genetic material in the supernatant.