Processes for Filtering Liquids Using Single Pass Tangential Flow Filtration Systems and Tangential Flow Filtration Systems with Recirculation of Retentate

摘要

Methods of filtering a liquid feed are disclosed. In one version, the method comprises passing a liquid feed through a single pass tangential flow filtration (SPTFF) system and recovering the retentate and permeate from the system in separate containers without recirculation through the SPTFF system. In another version, the method of filtering a liquid feed, comprises passing a liquid feed through a tangential flow filtration (TFF) system, recovering permeate and a portion of the retentate from the system in separate containers without recirculation through the TFF system, and recirculating the remainder of the retentate through the TFF system at least once. The methods can be performed using an SPTFF or a TFF system that comprises manifold segments to serialize the flow path of the feed and retentate without requiring diverter plates.

主权项

1. A method of filtering a liquid feed, comprising:
a) passing a liquid feed through a single pass tangential flow filtration (SPTFF) system that comprises:
a plurality of filtration modules that are fluidly connected, each filtration module comprising:
a manifold segment comprising a first manifold for receiving and carrying the feed into the filtration module, a second manifold for receiving and carrying retentate out of the filtration module, and a third manifold for receiving and carrying permeate through the filtration module; andone or more TFF capsules that are positioned on one or both faces of, and are fluidly connected to, the manifold segment;a feed inlet on the first filtration module in the system; anda retentate outlet on the last filtration module in the system, the SPTFF system having a liquid flow path that is parallel into the capsules in each filtration module and serial through the manifold segments in adjacent filtration modules by coupling of the first manifold in each manifold segment to the second manifold of an adjacent manifold segment, such that the retentate of one module serves as the feed for the next module, except that the first manifold in the first filtration module in the system is connected to the feed inlet and the second manifold in the last filtration module in the system is connected to the retentate outlet; and b) recovering the retentate and permeate from the system in separate containers without recirculation through the SPTFF system, thereby filtering the liquid feed.