Method of producing biologically active vitamin K dependent proteins by recombinant methods
摘要
The invention relates to commercially viable methods for producing biologically active vitamin K dependent proteins, particularly Factor IX. Factor IX is produced at a level of at least about 15 mg/L and is at least 25% biologically active. The method relies upon co-expression of one or more of paired basic amino acid converting enzyme (PACE), vitamin K dependent epoxide reductase (VKOR) and vitamin K dependent y-glutamyl carboxylase (VKGC) at a preferred ratio so that the vitamin K dependent protein is efficiently produced and processed by a recombinant cell.
申请公布号
AU2013211534(B2)
申请公布日期
2016.03.03
申请号
AU20130211534
申请日期
2013.08.02
申请人
UNIVERSITY OF NORTH CAROLINA AT CHAPEL HILL;CNJ HOLDINGS, INC.
发明人
STAFFORD, DARRELL W.;TAYLOR, JOHN R.;GRIFFITH, MICHAEL J.;DROHAN, WILLIAM N.