SYSTEMS AND METHODS FOR GENOTYPING SEED COMPONENTS

摘要

The invention provides methods for obtaining genetic material from plant embryos while preserving their viability as well as methods for performing a molecular analysis of plant embryos, particularly with small quantities of genetic material. The methods may include the steps of collecting shed cellular material from one or more plant embryos; obtaining DNA from the shed cellular material; performing a molecular analysis of the DNA; and germinating at least one of said one or more plant embryos. A further extension of this method includes determining whether to germinate and grow the embryo or to discard the embryo based on its genotype as part of a breeding process. Also provided are methods of genotyping embryos using embryo shed cellular material contained in or on agar and methods of analyzing plant embryonic tissue derived from microspores.

主权项

1. A method of analyzing plant embryonic tissue, said method comprising:
(a) collecting shed cellular material from plant embryonic tissue derived from a microspore by agitating the plant embryonic tissue in a non-destructive medium consisting essentially of water; (b) obtaining DNA from the collected shed cellular material; and (c) performing a molecular analysis of the DNA obtained in step (b).