ANALYSIS METHOD AND SYSTEM FOR ANALYZING A NUCLEIC ACID AMPLIFICATION REACTION

摘要

An analysis method is provided aimed at improving the linearity (precision and/or accuracy) of a quantification by a real-time nucleic acid amplification reaction RNR such as a polymerase chain reaction PCR over a wide range of analyte concentrations and/or limiting the effects of the presence of interfering substances by way of determining a quantification cycle number (Cq) of the RNR as the cycle number corresponding to an intersection of the growth curve with a combined threshold function (CTF) over the RNR cycle range comprising at least two different threshold levels, the quantification cycle number (Cq) being indicative of a quantitative and/or qualitative analysis result of a growth curve, the growth curve being indicative of the intensity of the fluorescence emission of an analyte for each amplification reaction cycle of the RNR over a RNR cycle range.

主权项

1. An analysis method for a real-time nucleic acid amplification reaction RNR, the method comprising:
acquiring an intensity of a fluorescence emission of an analyte for each amplification reaction cycle of the RNR; creating a growth curve indicative of the intensity of the fluorescence emission over a RNR cycle range, calculating a combined threshold function (CTF) over the RNR cycle range comprising at least two different threshold levels; determining a quantification cycle number (Cq) of the RNR, the quantification cycle number (Cq) being indicative of a quantitative and/or qualitative analysis result of the growth curve as the cycle number corresponding to an intersection of the growth curve with the combined threshold function (CTF).