Improved Gene Targeting and Nucleic Acid Carrier Molecule, In Particular for Use in Plants

摘要

The present invention relates to a nucleic acid carrier molecule, comprising the general formula M-S1-L-W—S2, wherein M is a first polypeptide specifically binding to a donor nucleic acid sequence to be transferred into an organelle of a cell, W is a second polypeptide specifically binding to a target nucleic acid sequence, wherein said target nucleic acid sequence is located in an organelle of a cell, L is missing or is linking group allowing M and W flexibility and semi-independence, and S1 and S2 independently of each other are missing or are a signal peptide sequence, and can be fused to M and W proteins either N- or C-terminally, wherein said donor nucleic acid sequence is brought into close proximity with said target nucleic acid sequence when both nucleic acid sequences are bound to said carrier molecule. The present invention furthermore relates to methods for recombinantly transforming a nucleic acid into an organelle in a cell, preferably a plant cell, employing said nucleic acid carrier molecule.

主权项

1. A nucleic acid carrier molecule, having the general formula
M-S1-L-W—S2, wherein M is a first polypeptide that specifically binds to a donor nucleic acid sequence to be transferred into an organelle of a cell, W is a second polypeptide that specifically binds to a target nucleic acid sequence, wherein said target nucleic acid sequence is located in an organelle of a cell, L is missing or is a linking group allowing M and W flexibility and semi-independence, and S1 and S2 independently of each other are missing or are a signal peptide sequence, wherein said donor nucleic acid sequence is brought into close proximity with said target nucleic acid sequence when both nucleic acid sequences are bound to said carrier molecule.