发明名称 |
IMPROVEMENT IN IMMOBILIZED MICROBIAL NITRILASE FOR PRODUCTION OF GLYCOLIC ACID |
摘要 |
The present invention is directed to a process for improving the specific activity of a dehydrated enzyme catalyst having nitrilase activity for hydrolysis of glycolonitrile to glycolic acid upon rehydration. In particular, a process is provided comprising pretreating an enzyme catalyst having nitrilase activity with glutaraldehyde, immobilizing the gutaraldehyde-preteated enzyme catalyst and chemically cross-linking the enzyme catalyst prior to dehydration. Upon rehydration, the enzyme catalyst exhibits improved specific nitrilase activity as compared to enzyme catalysts having nitrilase activity that are dehydrated and rehydrated without the processing described herein. |
申请公布号 |
EP2215226(B9) |
申请公布日期 |
2016.03.02 |
申请号 |
EP20080845012 |
申请日期 |
2008.10.31 |
申请人 |
E. I. DU PONT DE NEMOURS AND COMPANY |
发明人 |
DICOSIMO, ROBERT;BEN-BASSAT, ARIE |
分类号 |
C12N9/78;C12N11/00;C12P7/42 |
主分类号 |
C12N9/78 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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