发明名称 |
QUANTITATIVE ASSESSMENT OF HUMAN T-CELL REPERTOIRE RECOVERY AFTER ALLOGENEIC HEMATOPOIETIC STEM CELL TRANSPLANTATION |
摘要 |
A method and an apparatus are provided for determining T-cell repertoire recovery after allo-HSCT or identifying patients at high risk of infection. Combination of 5′-RACE PCR with deep sequencing was used to quantify TCR diversity in 33 individuals using a single oligonucleotide pair. Analysis of duplicate blood samples revealed highly reproducible detection of expanded TCR clonotypes. After 6 months, recipients of cord blood grafts without anti-thymocyte globulin therapy approximated the TCR diversity of healthy subjects, whereas recipients of T-cell-depleted peripheral blood stem cell grafts had a 28-fold and 14-fold lower CD4+ and CD8+ T-cell diversity, respectively. After 12 months, these differences had leveled out for the CD4+, but not the CD8+ T-cell compartment. |
申请公布号 |
EP2870264(A4) |
申请公布日期 |
2016.03.02 |
申请号 |
EP20130812533 |
申请日期 |
2013.07.03 |
申请人 |
SLOAN KETTERING INSTITUTE FOR CANCER RESEARCH |
发明人 |
PAMER, ERIC;VAN HEIJST, JEROEN W. J.;PERALES, MIGUEL-ANGEL;VAN DEN BRINK, MARCEL R. M. |
分类号 |
C12Q1/68;C07K14/725 |
主分类号 |
C12Q1/68 |
代理机构 |
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地址 |
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