发明名称 |
DNA polymerases with increased 3′-mismatch discrimination |
摘要 |
Disclosed are mutant DNA polymerases having increased 3′-mismatch discrimination relative to a corresponding, unmodified polymerase. The mutant polymerases are useful in a variety of disclosed primer extension methods. Also disclosed are related compositions, including recombinant nucleic acids, vectors, and host cells, which are useful, e.g., for production of the mutant DNA polymerases. |
申请公布号 |
US9273293(B2) |
申请公布日期 |
2016.03.01 |
申请号 |
US201414257653 |
申请日期 |
2014.04.21 |
申请人 |
Roche Molecular Systems, Inc. |
发明人 |
Reichert Fred;Bauer Keith;Myers Thomas W. |
分类号 |
C12N9/00;C12N9/12;C12Q1/68 |
主分类号 |
C12N9/00 |
代理机构 |
Kilpatrick Townsend & Stockton LLP |
代理人 |
Kilpatrick Townsend & Stockton LLP |
主权项 |
1. A recombinant nucleic acid encoding a DNA polymerase having at least 90% sequence identity to the amino acid sequence of SEQ ID NO:1, wherein the amino acid of the DNA polymerase corresponding to position 667 of SEQ ID NO:1 is E. |
地址 |
Pleasanton CA US |