| 发明名称 |
Methods for producing secreted polypeptides having biological activity |
| 摘要 |
The present invention relates to methods for producing a polypeptide having biological activity, comprising: (a) cultivating a fungal host cell in a medium conducive for the production of the polypeptide, wherein the fungal host cell comprises a first polynucleotide encoding the polypeptide operably linked to a second polynucleotide encoding a variant signal peptide or a variant prepropeptide; and (b) isolating the secreted polypeptide having biological activity from the cultivation medium. |
| 申请公布号 |
US9273104(B2) |
申请公布日期 |
2016.03.01 |
| 申请号 |
US201314081340 |
申请日期 |
2013.11.15 |
| 申请人 |
NOVOZYMES, INC.;NOVOZYMES A/S |
发明人 |
Bjornvad Mads;Yaver Debbie |
| 分类号 |
C07K7/00;C12N9/20;C07K14/37;C12N15/62;C12N15/81;C12P21/02;A61K38/00 |
主分类号 |
C07K7/00 |
| 代理机构 |
|
代理人 |
Starnes Robert |
| 主权项 |
1. An genetically engineered and isolated polynucleotide, which encodes a signal peptide variant consisting of amino acids 1 to 17 of SEQ ID NO: 2 without amino acids “SPIRR” wherein the amino acid residue 2 is substituted with Lys, and wherein the encoded signal peptide is directly fused in frame to the amino terminus of a mature polypeptide of interest which is encoded by said polynucleotide. |
| 地址 |
Davis CA US |
