Analyzing chemical and biological substances using nano-structure based spectral sensing

摘要

An integrated chromatography-immunoassay system for integrated chromatography-immunoassay system includes a chromatographic unit that receives labeled nano-structured probes comprising nano particles and antibodies attached to the nano particles, and a test membrane comprising coating antigens. The chromatographic unit allows the labeled nano-structured probes to diffuse there through and into the test membrane, wherein the antibodies on the nano particles are bound to the coating antigens. A laser device emits a laser light to illuminate the labeled nano-structured probes having the antibodies bound to the coating antigens on the test membrane. A spectral analyzer obtains a Raman spectrum from light scattered from the labeled nano-structured probes having the antibodies bound to the coating antigens on the test membrane, and to identify a spectral signature in the Raman spectrum associated with the antibody-antigen pair, which enables detection and identification of the antibody.

主权项

1. A method for identifying an antibody using an integrated chromatography-immunoassay system, comprising:
receiving, by a chromatographic unit, labeled nano-structured probes comprising nano particles, and Raman markers and antibodies attached to the nano particles; allowing the labeled nano-structured probes comprising the nano particles and the associated Raman markers and the antibodies to diffuse through a test membrane comprising coating antigens; allowing the antibodies on the associated nano particles with the associated Raman markers to bind to the coating antigens on the test membrane; illuminating the labeled nano-structured probes having the Raman markers, and the antibodies bound to the coating antigens on the test membrane by a laser light; obtaining a Raman spectrum from light scattered with a spectral analyzer from the labeled nano-structured probes having the antibodies bound to the coating antigens on the test membrane; and identifying a spectral signature related to the Raman markers in the Raman spectrum associated with the antibody-antigen pair, which enables detection and identification of at least one of the antibodies attached to the nano particles.