Method of and device for packing a chromatography column

摘要

A method and device for packing a chromatography column formed of one or more vibration devices attached to top and/or bottom flanges of the column. Media is added in one or more steps to the column, allowed to settle under the effects of gravity and then subjected to one or more treatments of vibration from the vibration devices until a suitably packed column is obtained. Liquid used to suspend the media while being placed into the column may be at least partially removed before or during the vibration step(s). The remaining liquid is then removed or replaced after the packing has been obtained.

主权项

1. A method of packing a chromatography column comprising the steps of:
a. providing a chromatography column having one or more vibration devices attached to its outer surface; b. forming a slurry with a media and a buffer solution; c. transferring the slurry into the column and immediately applying flow for 1 minute to settle the slurry in the column; d. activating the one or more vibration devices to generate vibrations for 1 minute following the settling of the slurry under flow for 1 minute; e. repeating flow for 1 minute followed by vibrations for 1 minute, until a packed bed of media is obtained.