Immunoassay product and process

摘要

The embodiments disclosed herein are directed to an apparatus useful in conducting detection of compounds on blotting membranes. The device is comprised of several layers including a porous support layer below the blotting membrane(s), a flow distributor above the blotting membrane(s) and optionally a well on the flow distributor to contain the liquid to the desired area and to allow for lower starting volumes of such liquid. Preferably, the flow distributor is a non-binding or low binding hydrophilic porous membrane such as a 0.22 micron membrane and the support layer is a grid or sintered porous material. The distributor and support are held together to form an envelope around the membrane(s). The use of a hinge, clips and other such devices is preferred in doing so.

主权项

1. A device for conducting immunoassays comprising a holder and a carrier for the holder,
a. the holder being formed of a material selected from the group consisting of plastic and paper, the holder comprising a frame having a perimeter edge, the frame configured to support a flow distributor; said flow distributor comprising a membrane attached to said frame and over which a blotting membrane can be positioned; a porous support comprising polypropylene mesh bound to said frame along a portion of said perimeter edge; and a sheet of paper laminated to said polyproylene mesh; and b. a carrier for the holder comprised of a top plate and a bottom plate, each having a width and a length, a top and bottom surface and a thickness between the top and bottom surfaces, an outer edge and at least one opening, the plates being of a length and width greater than the length and width of the holder, the top plate of the carrier having an opening substantially equal in width and length to the holder, the bottom surface of the top plate and the top surface of the bottom plate each having one or more seals in alignment with each other when the two plates are adjacent each other and the seals being arranged on each surface at a width and length greater than that of the opening of the top plate, but less than the outer dimensions of the holder, at least one of the plates has a seal formed adjacent the outer edge of the plate and outward of the seal of that plate.