Chinese hamster ovary cell lines

摘要

We provide a Chinese Hamster Ovary (CHO) cell which is capable of higher protein sialylation compared to a wild type Chinese Hamster Ovary cell, such as in the presence of functional GnT 1, in which the CHO cell is obtainable by selection with Ricinus communis agglutinin I (RCA-I).

主权项

1. A CHO cell comprising a mutation in the GnT I gene selected from the following mutations at the specified position of a GnT I nucleic acid sequence of GenBank Accession Number: AF343963: (a) a C to T transition at nucleic acid sequence position 1015; (b) a G to C transversion at nucleic acid sequence position 1300; (c) an A to C transversion at nucleic acid sequence position 638; (d) a C to G transversion at nucleic acid sequence position 784; (e) a T to A transversion at nucleic acid sequence position 811; (f) an insertion at nucleic acid sequence position 706 resulting in a frame shift from nucleic acid sequence position 236 of the encoded amino acid sequence; (g) a G to A transition at nucleic acid sequence position 246; (h) a G to A transition at nucleic acid sequence position 258; or (i) an A to T transversion at nucleic acid sequence position 859, in which the CHO cell further comprises a nucleic acid encoding a protein of interest comprising a glycoprotein in an expression vector and a nucleic acid encoding a functional GnT I sequence.