Methods for treatment of bacterial infections

摘要

The peptidoglycan layer is a vital component of the bacterial cell wall, which comprises 4→3 and 3→3 transpeptide cross-linkages, the formation of which are catalyzed by D,D- and L,D-transpeptidases, respectively. Methods for the treatment of bacterial infections with agents that inhibit L,D-transpeptidases, either alone or in combination with D,D-transpeptidase inhibitors, are provided herein. Also provided are methods for the treatment of chronic stage tuberculosis with agents that inhibit enzyme with L,D-transpeptidase activity.

主权项

1. A method of identifying a LdtMt2 L, D-transpeptidase enzyme ligand, comprising:
contacting LdtMt2 L, D-transpeptidase having a first mass with a test agent in vitro; and detecting formation of an LdtMt2 L, D-transpeptidase-adduct by mass spectrometry, wherein the formation of an adduct is evidenced by an overall increase in the first mass of the enzyme, thereby identifying a LdtMt2 L, D-transpeptidase enzyme ligand.