Pseudoinfectious flavivirus and uses thereof

摘要

The present invention discloses a replication-deficient pseudoinfective virus belonging to the Flaviviridae family that lack the capsid gene, where the replication-deficient pseudoinfective virus propagates only in cells expressing the capsid or capsid, prM and envelope protein of the flavivirus. The present also discloses the method of producing such viruses on a large scale and the use of these pseudoinfective viruses as vaccines for preventing diseases caused by infections of humans or animals by the viruses belonging to this family.

主权项

1. A replication-deficient pseudoinfectious virus comprising:
a mutant flavivirus genome having a deletion of nucleotide sequence encoding amino acids 26 to 93, 31 to 93, 31 to 100, or 26 to 100 of the flavivirus capsid protein, wherein the deletion mutant genome cannot produce capsid-containing viral particles in a cell that does not express a capsid protein, and wherein the deletion does not disrupt the maturation of prM protein or the RNA sequence required for genome cyclization.