Placental tissue grafts

摘要

Described herein are tissue grafts derived from the placenta. The grafts are composed of at least one layer of amnion tissue where the epithelium layer has been substantially removed in order to expose the basement layer to host cells. By removing the epithelium layer, cells from the host can more readily interact with the cell-adhesion bio-active factors located onto top and within of the basement membrane. Also described herein are methods for making and using the tissue grafts. The laminin structure of amnion tissue is nearly identical to that of native human tissue such as, for example, oral mucosa tissue. This includes high level of laminin-5, a cell adhesion bio-active factor show to bind gingival epithelia-cells, found throughout upper portions of the basement membrane.

主权项

1. A method for preparing a dehydrated tissue graft, comprising the steps:
a) obtaining a placenta comprising an amnion and a chorion; b) separating the amnion from the chorion; c) washing and cleaning the amnion and optionally the chorion, wherein the amnion comprises an epithelial cellular side and a fibroblast cellular side; d) substantially removing all of the epithelial cells from the epithelial cellular side of the amnion while retaining fibroblast cells in the fibroblast cellular side; e) forming a multilayered graft by placing the amnion or the chorion on a surface to form a first layer and then placing one or more additional layers over the first layer to produce a multilayered tissue graft, wherein at least one layer comprises the amnion with the epithelial cells substantially removed while retaining fibroblast cells, and wherein the one or more additional layers is not Wharton's jelly; and f) dehydrating the multilayered tissue graft.