发明名称 |
NOVEL SELECTION VECTORS AND METHODS OF SELECTING EUKARYOTIC HOST CELLS |
摘要 |
The disclosure pertains to an expression vector or a combination of at least two expression vectors comprising: a) a polynucleotide encoding a mutated folate receptor as selectable marker, wherein the mutated folate receptor has a decreased folate binding affinity compared to the wild type folate receptor; b) at least one polynucleotide encoding a polypeptide of interest, wherein when said expression vector or combination of at least two expression vectors is introduced into a host cell, the polypeptide of interest is secreted from said host cell. Also provided are suitable host cells, selection methods and methods for producing polypeptides with high yield. |
申请公布号 |
SG11201510625T(A) |
申请公布日期 |
2016.02.26 |
申请号 |
SGT11201510625 |
申请日期 |
2014.07.29 |
申请人 |
NOVARTIS AG |
发明人 |
ASSARAF, YEHUDA G.;JOSTOCK, THOMAS;KNOPF, HANS-PETER |
分类号 |
C07K14/705;C12N15/65;C12N15/69;C12N15/85 |
主分类号 |
C07K14/705 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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