| 摘要 |
Disclosed is a method of producing squalene by a genetically altered yeast, said method comprising increasing activity or expression of squalene synthase by a yeast cell by introducing into the yeast cell (i) one or more mutations into a squalene synthase gene of the yeast using a gene repair oligonucleobase or (ii) an exogenous squalene synthase gene, thereby genetically altering the yeast; and selecting a yeast cell expressing the squalene synthase having the one or more mutations or selecting a yeast cell expressing the exogenous squalene synthase gene, wherein said genetically altered yeast produces increased quantities of squalene as compared to yeast expressing squalene synthase which lacks the one or more mutations or yeast lacking the exogenous squalene synthase gene. |
