发明名称 |
ELECTROCHEMICAL DETECTION OF MICROORGANISMS |
摘要 |
The present invention provides a method for determining the presence of a microorganism in a sample using an electrochemically active reporter, wherein the method comprises (a) contacting the sample with an electrochemically active reporter, wherein the electrochemically active reporter is a conjugate comprising a sugar moiety and a redox active reporter moiety that are covalently linked such that the covalent bond can be enzymatically cleaved in the presence of the microorganism by an enzyme expressed by the microorganism, wherein the redox active reporter moiety is selected from the group consisting of resorufin and compounds of formula (I) as defined herein, under conditions that allow enzymatic cleavage of the covalent bond between the sugar moiety and the redox active reporter moiety and reduction of the redox active report moiety in the presence of the microorganism; (b) electrochemically determining the released redox active reporter moiety; and (c) determining the presence of the microorganism and, optionally, number of the microorganisms in the sample based on the determined released redox active reporter moiety. Also encompassed are the electrochemically active reporters used in the described methods and their use for determination of the presence of microorganisms in a sample. |
申请公布号 |
WO2016028233(A1) |
申请公布日期 |
2016.02.25 |
申请号 |
WO2015SG50274 |
申请日期 |
2015.08.24 |
申请人 |
NANYANG TECHNOLOGICAL UNIVERSITY;NATIONAL UNIVERSITY OF SINGAPORE |
发明人 |
HINKS, JAMIE;HAN, JING YING EVELINA;LOO, SAY CHYE JOACHIM;WUERTZ, STEFAN |
分类号 |
C12Q1/54;C07H15/203;C07H17/00;C12Q1/04;C12Q1/06;G01N27/26 |
主分类号 |
C12Q1/54 |
代理机构 |
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代理人 |
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地址 |
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