COMPOSITIONS AND ASSAYS FOR DETERMINING CELL VIABILITY

摘要

Provided are compositions and methods useful in evaluation of cell health and metabolism, cell viability, proliferation, and the effects of compounds on these qualities. The assays provided are rapid, robust, nontoxic and suitable for use with high throughput devices.

主权项

1. A method for assessing a metabolic activity of a cell in a cell culture, the method comprising adding a reagent solution comprising resazurin, a buffer and an aqueous solvent to the cell culture, wherein the resazurin is initially present in the oxidized state and the assessing of metabolic activity is performed by measuring for production of the reduced state of the resazurin within 60 minutes of adding the reagent solution to the cell culture.