PROCESS FOR THE PREPARATION OF ERYTHROCYTES LOADED WITH ONE OR MORE SUBSTANCES OF PHARMACEUTICAL INTEREST AND SO OBTAINED ERYTHROCYTES

摘要

The present invention relates to a process for preparing erythrocytes loaded with one or more substance of pharmaceutical interest. The present invention is also directed to loaded erythrocytes thus obtained and pharmaceutical compositions comprising said population of loaded erythrocytes as well as therapeutic application thereof, in particular in the treatment of Ataxia telangiectasia.

主权项

1. A process for preparing erythrocytes loaded with one or more active ingredients comprising the following steps:
a) swelling the erythrocytes using a first hypotonic solution; b) further swelling the erythrocytes obtained in step a), without reaching the lysis, using a second hypotonic solution more hypotonic than the first hypotonic solution; c) concentrating the erythrocytes obtained in step b); d) placing the concentrated erythrocytes in contact with a lysing solution comprising one or more substances of pharmaceutical interest, and subsequently e) adding a sealing solution for the purpose of obtaining a population of erythrocytes loaded with said one or more active principles.