| 发明名称 |
Culture Medium And Method For Inducing Differentiation of Pluripotent Stem Cells To Hepatoblasts |
| 摘要 |
Provided are a method of inducing differentiation of pluripotent stem cells, such as induced pluripotent stem cells (hereinafter abbreviated as iPS cells), to hepatocytes in a short period of time, and a substance to be used in the method. Specifically, provided are a method of producing a cell culture formed substantially of hepatoblasts, the method including culturing pluripotent stem cells, such as iPS cells, in a culture medium having a composition shown in Table 1 below, and a culture medium for inducing differentiation of pluripotent stem cells into hepatoblasts, which has a composition shown in Table 1 below. |
| 申请公布号 |
US2016053230(A1) |
申请公布日期 |
2016.02.25 |
| 申请号 |
US201514749715 |
申请日期 |
2015.06.25 |
| 申请人 |
National University Corporation Chiba University |
发明人 |
Tomizawa Minoru |
| 分类号 |
C12N5/071 |
主分类号 |
C12N5/071 |
| 代理机构 |
|
代理人 |
|
| 主权项 |
1. A method of producing hepatoblasts from pluripotent stem cells,
comprising culturing pluripotent stem cells in a culture medium having a composition shown in Table 1 below.TABLE 1HDI (1 L)inorganic saltsCaCl2,2H2O0.185gMgCl2,6H2O0.203gMgSO4(anhyd)0.098gKCl0.4gKH2PO40.06gNaCl7.915gNa2HPO40.19gamino acidsL-alanine0.225gL-asparagine,H2O0.25gL-cysteine0.12gglycine0.2gL-histidine,HCl,H2O0.25gL-isoleucine0.25gL-leucine0.125gL-lysine,HCl0.075gL-methionine0.075gL-phenylalanine0.125gL-serine0.2gL-threonine0.3gL-tryptophan0.02gL-valine0.1gOthersphenol red, Na0.01gNaHCO32.745gMEM vitamin solution (100 X)10mlknockout serum replacement100mlglutamine0.3gornithine0.169ggalactose0.9goncostatin M0.02gFPH13.88gM50054100mgnon-essential amino acids10mlsodium pyruvate10mlnicotinamide1.2gproline0.03g |
| 地址 |
Chiba JP |
