INJECTOR SAMPLE DILUTION FOR A LIQUID CHROMATOGRAPHY SYSTEM

摘要

Described are a method and a system for diluting a sample at a location of injection in a liquid chromatography system. The method includes loading a sample into a first fluid channel, separating a flow of a mobile phase into a first flow in the first fluid channel and a second flow in a second fluid channel, and combining the sample that is displaced from the first fluid channel and the mobile phase exiting the second fluid channel at the location of injection into the system flow to thereby generate a diluted sample in the system flow. The dilution ratio of the diluted sample is responsive to the flow rates of the first and second flows. Advantageously, the flow rates can be changed by changing the flow restriction of one of the fluid channels. Thus providing the proper flow restriction enables a user to obtain a desired dilution ratio.

主权项

1. A method of diluting a sample in a liquid chromatography system, the method comprising:
loading a sample into a first fluid channel; separating a flow of a mobile phase of a liquid chromatography system into a first flow in the first fluid channel and a second flow in a second fluid channel, the first flow and the second flow having a first flow rate and a second flow rate, respectively, wherein the flow of mobile phase in the first fluid channel displaces the sample in the first fluid channel; and combining the sample exiting the first fluid channel and the mobile phase exiting the second fluid channel at a location of injection into the system flow to thereby generate a diluted sample in the system flow, wherein a dilution ratio of the diluted sample is responsive to the first flow rate and the second flow rate.