发明名称 |
METHOD FOR COATING A SOLID SUPPORT |
摘要 |
The present invention relates to a method for coating a solid support which is suitable for being used in cell culture applications. The method comprises a step in which a solution of a carrier protein, which comprises attached thereto or as a part of its amino acid sequence at least one cell function- modulating peptide sequence, is heated to at least 50°C and incubated same under conditions which result in the formation of soluble carrier protein aggregates comprising between 2 and 1000 protein molecules. Subsequently, the protein aggregates are brought into contact with the solid support under conditions that allow for the non-covalent adsorption of the aggregates to the support. The invention also relates to a coated solid support having non-covalently adsorbed on its surface at least one of the above-mentioned aggregate of carrier proteins aggregates. |
申请公布号 |
WO2016026932(A1) |
申请公布日期 |
2016.02.25 |
申请号 |
WO2015EP69150 |
申请日期 |
2015.08.20 |
申请人 |
EPPENDORF AG |
发明人 |
PLUESTER, WILHELM;REMACLE, JOSE;ALEXANDRE, ISABELLE;PLENNEVAUX, CHRISTELLE;DE LONGUEVILLE, FRANCOISE |
分类号 |
G01N33/543;C12M1/00;C12M1/32 |
主分类号 |
G01N33/543 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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