ARS INDUCED GENE AMPLIFICATION

摘要

The present invention relates to the use of an Autonomously Replicating Sequence (ARS) to modulate gene amplification in a eukaryotic cell. Preferably, an ARS sequence is introduced in the neighborhood of a gene of interest, and the cells are grown under selective conditions, requiring a high expression of the gene of interest. Alternatively, an existing ARS sequence is deleted to increase the genetic stability of a certain DNA region.