发明名称 |
METHOD FOR IN VITRO PRODUCTION OF THREE-DIMENSIONAL VITAL CARTILAGE OR BONE TISSUE |
摘要 |
In vitro preparation of three-dimensional, living cartilage or bone tissue comprising culturing human or animal cartilage, bone or mesenchymal cells in vessels that have a hydrophobic surface and a tapering base, is new. In vitro preparation of three-dimensional, living cartilage or bone tissue comprises culturing human or animal cartilage, bone or mesenchymal cells in vessels that have a hydrophobic surface and a tapering base Culture is done until cell aggregates are formed, under stationary, submerged conditions. The culture contains at least 40% extracellular matrix (ECM) and has differentiated cells embedded in ECM. The aggregates include an outer region in which proliferation- and migration-competent cells are present. Independent claims are also included for the following: (a) production of cartilage and bone tissue; and (b) therapeutic composition containing the cartilage and bone tissue. - ACTIVITY : Antiarthritic; antirheumatic. No biological data provided. - MECHANISM OF ACTION : Cell replacement. No biological data provided. |
申请公布号 |
EP1265986(B1) |
申请公布日期 |
2016.02.24 |
申请号 |
EP20010913858 |
申请日期 |
2001.03.09 |
申请人 |
CO.DON AKTIENGESELLSCHAFT |
发明人 |
LIBERA, JEANETTE;ANDERER, URSULA;FRITSCH, KARL-GERD;JOSIMOVIC-ALASEVIC, OLIVERA |
分类号 |
C12N5/02;A61K35/12;A61K35/32;A61P19/00;C12N5/077 |
主分类号 |
C12N5/02 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|