| 摘要 |
The present invention relates to the field of DNA diagnosis, in particular, to detection of single nucleotide polymorphisms (SNPs). Single nucleotide polymorphisms play an important role in many diseases having a genetic component, e.g., in multiple sclerosis. The invention provides an electrochemical method of detection of SNP, which exploits the different temperature optima of detection of DNA of varying sequences labeled with a redox marker such as osmium tetroxide bipyridine, wherein the detection is preferably carried out at a directly heated electrode. The invention also relates to kits for carrying out said method, and a method of diagnosing a disease associated with a SNP. |
