发明名称 |
A METHOD AND A DEVICE FOR CROSS-TALK CORRECTION OF MEASURED INTENSITIES |
摘要 |
A method for cross-talk correction of intensities measured on mutually separate detection wavelength bands is presented. Each detection wavelength band relates to one of analyte-specific probe-populations contained by a sample to be analyzed. Each probe-population is capable of emitting a first signal component and a second signal component whose spectra have maxima at different wavelengths and at least the first signal component is dependent on presence of analyte detectable with that probe-population. Cross-talk corrected intensities are computed on the basis of a) the intensities measured on the detection wavelength bands, b) a value indicative of intensity occurring on an auxiliary wavelength band outside the detection wavelength bands and at least partially caused by the second signal components, and c) pre-determined cross-talk parameters. For example in con-junction with FRET-based assays, the dependency of a background signal on the percentage of hybridized probes can be taken into account in the cross-talk correction. |
申请公布号 |
EP2986737(A1) |
申请公布日期 |
2016.02.24 |
申请号 |
EP20140721911 |
申请日期 |
2014.04.08 |
申请人 |
WALLAC OY |
发明人 |
LAITALA, VILLE;HARJU, RAIMO |
分类号 |
C12Q1/68;G01N21/27;G01N21/64 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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