Polypeptides having nucleic acid binding activity and compositions and methods for nucleic acid amplification

摘要

Polypeptides having nucleic acid binding activity are provided. Methods of using polypeptides having nucleic acid binding activity are provided. Fusion proteins and methods of using fusion proteins are provided. Fusion proteins comprising a polymerase and a nucleic acid binding polypeptide are provided. Fusion proteins comprising a reverse transcriptase and a nucleic acid binding polypeptide are provided. Methods are provided for amplifying a nucleic acid sequence using a fusion protein comprising a nucleic acid binding polypeptide and a polymerase. Methods are provided for amplifying a nucleic acid sequence using a fusion protein comprising a nucleic acid binding polypeptide and a reverse transcriptase.

主权项

1. A recombinant fusion protein comprising:
(a) a first polypeptide having nucleic acid binding activity and comprising an amino acid sequence having at least 90% identity to the amino acid sequence set forth in SEQ ID NO: 1, 4, or 6; and (b) a second polypeptide having DNA polymerase activity and/or reverse transcriptase activity.