Cell culture insert

摘要

A cell culture device allows co-cultivation on both sides of a membrane and has an outer tubular body with upper end having an opening and lower end closed by the membrane, an inner surface and an outer surface, which can be colonized with biological material, a complementary tubular body whose inner diameter fits onto the outer diameter of the lower end and a hanger component with upper end and a lower end. A flange extends from the upper end and the outer diameter component fits the inner diameter of the outer tubular body. The outer tubular body in a first step is connected to the complementary tubular body to form a standing insert for a first cell cultivation, and in a second step wherein the outer tubular body is disengaged from the complementary tubular body and is attached to the hanger component for a second cell cultivation.

主权项

1. A cell culture device comprising:
an outer tubular body (1) having an upper end (1a) and a lower end (1b), the upper end (1a) comprising an opening and the lower end (1b) being closed by a membrane (4), the membrane having an inner surface (4a) and an outer surface (4b) which can both be colonized with biological material, characterized in that the cell culture device further comprises: a complementary tubular body (2), whose inner diameter fits, at least partly, onto the outer diameter of the lower end (1b) of the outer tubular body (1), and a hanger component (3) having an upper end (3a), a lower end (3b), an axial passage there through, and an outer surface; wherein the axial passage is open at least at the lower end (3b) of the hanger component (3); wherein at least one flange (5) extends sideways from the upper end (3a) of the hanger component (3); wherein at least part of the outer surface of the lower end (3b) of the hanger component (3) has a shape complimentary to an inner surface of the outer tubular body (1), and wherein the lower end (3b) of the hanger component and the inner surface of the outer tubular body (1) collectively form a connection means adapted to reversibly axially connect the hanger component (3) and the tubular body (1); wherein said outer tubular body (1) is shaped for reversible connection to the complementary tubular body (2) to form a standing insert for a first cell cultivation, and wherein the hanger component (3), through said connection means, is able to fully support the tubular body (1) in a position hanging below the hanger component (3) for a second cell cultivation.