STXBP1 overexpressing mouse and its uses in screening of treatments for neuropsychiatric illness

摘要

A non-human transgenic animal having a polynucleotide encoding an STXBP1 polypeptide, which polynucleotide is operably linked to a promoter, wherein said transgenic animal has greater than wild-type expression of the STXBP1 polypeptide in at least one brain region, as well as related vectors, methods of producing transgenic animals, in vitro and in vivo screening methods for potential therapeutic agents, and methods for treating and diagnosing neuropsychiatric illness are disclosed.

主权项

1. A transgenic mouse whose genome comprises a polynucleotide encoding a syntaxin-binding protein 1 (STXBP1) polypeptide having at least 90% sequence identity with SEQ ID NO: 2 operably linked to an excitatory amino acid transporter 3 (EAAT3) promoter, wherein said transgenic mouse has greater than wild-type expression of the STXBP1 polypeptide in at least its brain cortex, and wherein the transgenic mouse exhibits one or more behaviours selected from the group consisting of reduced motor activity in an open field test, reduced time spent in open arms of an elevated plus maze, reduced social interaction, increased recognition index in a novel object recognition task, and decreased prepulse inhibition of startle response.