Removal of ions from survey scans using variable window band-pass filtering to improve intrascan dynamic range

摘要

Systems and methods are used to band-pass filter ions from a mass range. A full spectrum is received for a full scan of a mass range using a tandem mass spectrometer. A mass selection window of the full spectrum is selected and a set of tuning parameter values is selected. The tandem mass spectrometer is instructed to perform a scan of the mass selection window using the set of tuning parameter values. A spectrum is received for the scan from the tandem mass spectrometer. A band-pass filtered spectrum is created for the mass range that includes values from the spectrum for the mass selection window of the mass range. Systems and methods are also used to band-pass filter ions from two or more mass selection windows across the mass range and to filter out ions from a mass selection window between two band-pass mass selection windows.

主权项

1. A system for band-pass filtering ions from a mass range, comprising:
a tandem mass spectrometer that includes a mass analyzer that can analyze precursor ions or fragment ions using different sets of tuning parameter values; and a processor in communication with the tandem mass spectrometer that
instructs the tandem mass spectrometer to perform a precursor ion survey scan of a mass range, producing a full precursor ion spectrum for the mass range,receives the full precursor ion spectrum from the tandem mass spectrometer,from the full precursor ion spectrum, identifies for removal precursor ions that produce saturation in a detector sub-system of the mass analyzer,across the mass range, selects non-overlapping precursor mass selection windows with variable window widths based on the precursor ions identified for removal, wherein each precursor mass selection window is selected either to include a precursor ion identified for removal and to prevent precursor ions from being fragmented or is selected to not include a precursor ion identified for removal and to allow precursor ions to be fragmented and allow resulting fragmented ions to be mass analyzed,instructs the tandem mass spectrometer to fragment and analyze fragment ions of only precursor mass selection windows that are selected to not include a precursor ion identified for removal using a set of tuning parameter values for the detection sub-system, producing a plurality of fragment ion spectra, andreceives the plurality of fragment ion spectra from the tandem mass spectrometer and creates a band-pass filtered fragment ion spectrum from the plurality of fragment ion spectra.