Deconvolution method

摘要

The present invention relates generally to novel applications in combating infectious disease, cancer, allergy and autoimmune diseases. In one aspect, the invention relates to identifying one or more protein binding moieties of interest. In another aspect, the present invention relates to identifying one or more candidate vaccines.

主权项

1. A method for identifying an antibody or antigen binding fragment thereof comprising the steps of:
(a) screening a library of antibodies or antigen binding fragments against at least one immunogen; (b) obtaining a plurality of antibodies or antigen binding fragments that are specific for the immunogen; (c) dividing the plurality of antibodies or antigen binding fragments into at least a first pool and a second pool, wherein each pool contains at least one antibody or antigen binding fragment; (d) testing said antibodies or antigen binding fragments from said first pool for function in an vitro assay; (e) dividing the antibodies or antigen binding fragments from said first pool into two or more sub-pools, to the extent function in an vitro assay is observed in step (d), wherein each sub-pool contains at least one antibody or antigen binding fragment; (f) testing said antibodies or antigen binding fragments from said first and second sub-pools of said first pool for function in an vitro assay; and (g) identifying at least one antibody or antigen binding fragment from step (f) that are functional in an vitro assay.