Electrochemical impedance-based label-free and rapid biosensor for select bodily fluid biomolecule levels. Monoclonal antibodies to of biomolecule such as Cortisol were covalently attached to a 16-mercaptohexadecanoic acid functionalized gold working electrode using zero-length crosslinkers N-(3-dimethylaminopropyl)-N- ethylcarbodiimide and 10mM N-hydroxysulfosuccinimide. Cortisol was detected in phosphate buffered saline (simulated tear fluid) using a simple ferrocyanide reagent with a lower limit of detection of 18.73pM and less than 10% relative standard deviation.
申请公布号
CA2956696(A1)
申请公布日期
2016.02.18
申请号
CA20152956696
申请日期
2015.07.27
申请人
ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY