Method and Optical Arrangement for Manipulating and Imaging a Microscopic Sample

摘要

The invention relates to a method in which a sample is manipulated with manipulation light, and in which the sample is imaged by means of the SPIM technique under illumination with illumination light, in particular excitation light for fluorescence excitation, in the form of an illumination light sheet. The method is notable for the fact that both the manipulation light and the illumination light are focused by the same objective that is arranged in an objective working position, or by different objectives that are brought successively into an objective working position; and that the manipulation light and/or the illumination light, after passing through the objective, is diverted by means of a diverting device in such a way that it propagates at an angle different from zero degrees with respect to the optical axis of the objective.

主权项

1. A method in which a sample is manipulated with manipulation light, comprising:
imaging the sample using a single plane illumination microscopy SPIM technique under illumination with illumination light such as excitation light for fluorescence excitation, in the form of an illumination light sheet; focusing both the manipulation light and the illumination lighta. by a same objective arranged in an objective working position, orb. by different objectives brought successively into the objective working position; and
diverting at least one of the manipulation light or the illumination light, after passing through the objective, by means of a diverting device in such a way as to propagate the manipulation light or illumination light at an angle different from zero degrees with respect to an optical axis of the objective.