DETECTION OF NUCLEIC ACIDS

摘要

Provided herein is technology relating to detecting and identifying nucleic acids and particularly, but not exclusively, to compositions, methods, kits, and systems for detecting, identifying, and quantifying target nucleic acids with high confidence at single-molecule resolution.

主权项

1. A complex for detecting a target nucleic acid, the complex comprising:
a) a target nucleic acid comprising a first region and a second region; b) a capture probe comprising a target binding region hybridized to the first region of the nucleic acid to form a thermodynamically stable duplex; and c) a detectably labeled query probe that hybridizes repeatedly to the second region of the target nucleic acid with a kinetic rate constant koff that is greater than 0.1 min−1 and/or a kinetic rate constant kon that is greater than 0.1 min−1.