COMPOSITIONS, METHODS AND KITS FOR DIAGNOSIS OF LUNG CANCER

摘要

Methods are provided for identifying biomarker proteins that exhibit differential expression in subjects with a first lung condition versus healthy subjects or subjects with a second lung condition. Also provided are compositions comprising these biomarker proteins and methods of using these biomarker proteins or panels thereof to diagnose, classify, and monitor various lung conditions. The methods and compositions provided herein may be used to diagnose or classify a subject as having lung cancer or a non-cancerous condition, and to distinguish between different types of cancer (e.g., malignant versus benign, SCLC versus NSCLC).

主权项

1. A method of determining the likelihood that a pulmonary nodule in a subject is not lung cancer, comprising:
(a) contacting a blood sample obtained from the subject with a proteolytic enzyme to produce peptide fragments from a panel of proteins present in the blood sample, wherein the panel comprises ALDOA, FRIL, LG3BP, TSP1, and COIA1; (b) combining the produced peptide fragments from the panel from step (a) with labeled, synthetic peptide fragments which correspond to the produced peptide fragments from the panel; (c) performing selected reaction monitoring mass spectrometry to measure the abundance of the peptide fragments from step (b); (d) calculating a probability of lung cancer score based on the peptide fragment measurements of step (c); and (e) ruling out lung cancer for the subject if the score in step (d) is lower than a pre-determined score.