RADIOTRACER IMAGING USING SODIUM IODIDE SYMPORTER POLYPEPTIDES

摘要

This document provides methods and materials involved in radiotracer imaging using NIS polypeptides. For example, methods and materials for performing imaging techniques that increase the detection sensitivity and resolution of radiotracers localized by NIS reporter gene expression and/or decrease background signals that can be attributed to endogenously expressed NIS polypeptides are provided.

主权项

1. A method of imaging an animal comprising cells expressing a NIS transgene, wherein said method comprises:
(a) administering two or more gamma-emitting NIS radiotracers to said animal, (b) collecting imaging data during a single imaging session using at least two energy windows that distinguish the gamma emissions of said two or more radiotracers to obtain at least two gamma emission datasets, (c) subtracting one of said at least two gamma emission datasets from another of said at least two gamma emission datasets to obtain a resulting dataset capable of being used to generate subtraction images for visual or other analysis.