发明名称 |
METHOD OF ESTIMATING THE AMOUNT OF A METHYLATED LOCUS IN A SAMPLE |
摘要 |
A method of estimating the amount of a methylated locus is provided. In certain embodiments the method comprises: digesting a nucleic acid sample that contains both unmethylated and methylated copies of a genomic locus with an MspJI family member to produce a population of fragments that are in the range of 20-40 nucleotides in length, ligating adaptor sequence A and adaptor sequence B to the respective ends of a target fragment of sequence X, and quantifying the amount of ligation products of formula A-X-B. A kit for performing the method is also provided. |
申请公布号 |
WO2016024182(A1) |
申请公布日期 |
2016.02.18 |
申请号 |
WO2015IB55799 |
申请日期 |
2015.07.31 |
申请人 |
VANADIS DIAGNOSTICS |
发明人 |
DAHL, CARL OSCAR FREDRIK;ERICSSON, JOHN OLOF;BANÉR, JOHAN |
分类号 |
C12Q1/68 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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地址 |
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